Basic Information
Description
The mouse Met gene was replaced by human MET coding sequence in B-hMET MC38 cells. Human MET is highly expressed on the surface of B-hMET MC38 cells.
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Gene targeting strategy
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Gene targeting strategy for B-hMET MC38 cells. The exogenous promoter and human MET coding sequence was inserted to replace part of murine exon 3. The insertion disrupts the endogenous murine Met gene, resulting in a non-functional transcript.
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Protein expression analysis
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MET expression analysis in B-hMET MC38 cells by flow cytometry. Single cell suspensions from wild-type MC38 and B-hMET MC38 cultures were stained with species-specific anti-MET antibody. Mouse MET was not detected on the surface of MC38 cells. Human MET was detected on the surface of B-hMET MC38 cells but not wild-type MC38 cells. The 2-D07 clone of B-hMET MC38 cells was used for in vivo experiments
