When running a study, there are often intermediate experiments that require tracking or monitoring the expression of certain genes of interest. Analogous to general knockin/knockout mice, we help researchers insert reporter genes into precise predetermined regions of the genome under the control of certain promoters.
One way to “tag” certain genes is through introducing an exogenous gene that is easily detected, whether through a color change based on metabolic compounds (like LacZ), through fluorescence (such as GFP), or through protein tags (such as FLAG). Tag or Reporter genes thus often carry a function that is useful for a specific experimental design, which will help dictate which specific locus to insert in.
As disease models, these mouse models can be used to mimic genetic diseases when mutation(s) are introduced. Furthermore, gene expression when genes are tagged with various proteins (e.g. EGFP, mRFP, mCherry, YFP, LacZ, and Flag etc). To generate a knockin mouse, a DNA fragment of interest is inserted at the desired location in the genome. This modality also allows for a variety of models to be created including point mutant, reporter (GFP), tagged (FLAG), Cre, and humanized mice.
Genes tagged with EGFP, YFP, LacZ, Flag, mCherry and other sequences are useful for monitoring gene expression. Reporter gene mouse models are used to construct phylogenetic trees for cell development studies. Replacement of an endogenous gene with a reporter can simultaneously achieve gene knockout and knockin in the same mouse model.
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