The high-fat methionine-choline-deficient diet (HFMCD) is a classic model for NASH induction, which consists of a 60 kcal% fat, methionine and choline-deficient diet. Both methionine and choline can promote the delivery of fat from the liver through the blood in the form of phospholipids, improve the utilization of fatty acids in the liver, and prevent the abnormal accumulation of liver fat. HFMCD-induced NASH mice display increased liver injury, hepatic steatosis, and fibrosis accompanied by increased NAS scores.
