Immunodeficient (B-NDG) Mice

The immunodeficient B-NDG mouse model (NOD-Prkdcscid IL2rgtm1/Bcgen) was independently designed and generated by Biocytogen. B-NDG mice are generated by deleting the IL2rg gene from NOD-scid mice with severe immunodeficiency phenotype. Lacking mature T cells, B cells or functional NK cells, and displaying cytokine signaling deficiencies, this mouse model has the highest degree of immunodeficiency and thus is most suitable for engraft and growth of human hematopoietic stem cells (HSCs), peripheral blood mononuclear cells (PBMCs) and human tumor cells or tissues.

  • NOD-scid or NSG mice (non-obese diabetes, severe combined immunodeficiency) genetic background: mice of NOD genetic background and with Prkdc (protein kinase DNA-activated catalytic) knockout. Functional T cells, B cells and the complement system in these mice are lost, and the activity of NK cells is greatly weakened.
  • IL2rg null: the gamma chain of Interleukin-2 receptor (IL-2R γc, also called CD132) is on the mouse X chromosome, and is the common receptor subunit of cytokines IL2, IL-4, IL-7, IL-9, IL-15 and IL-21 with important immune functions. After IL2r is knocked out, mouse immunity function is greatly weakened, activities of NK cells, which are almost completely lost.
  • Prkdc null (DNAPK, scid): Prkdc (protein kinase DNA-activated catalytic) null mutation is characterized by significantly deficient of functional T cells and B cells, and an absence of lymphocytes, recapitulating severe combined immunodeficiency (scid) in human patients.

Advantages of B-NDG Mice

  • Model currently holds the highest degree of immunodeficiency among all immunodeficiency models
  • Longer lifespan than NOD-scid or NSG mice; 1.5 years on average
  • Minimal to absent rejection of human-derived cells and tissues
  • More efficient for CDX model generation
  • No B lymphocyte leakage

Major Applications

  • Human-derived cell or tissue engraftment
  • Tumor and tumor stem cell research
  • ES and iPS cell research
  • Hematopoiesis and immunology studies
  • Human infectious disease studies
  • Development of humanized models

B-NDG Mice

Product Product Name Catalog Number
B-NDG Mice B-NDG 110586

B-NDG-Based Mouse Models

Product Product No Background
B-NDG B2m KO mice plus 110601 B-NDG
B-NDG HLA-A2.1 mice 110599 B-NDG
B-NDG hCSF1/hTHPO mice 120609 B-NDG
B-NDG hIL15 mice 110600 B-NDG
B-NDG hIL6 mice 110596 B-NDG
B-NDG hSIRPA mice 110604 B-NDG
B-NDG hSIRPA/hCD47 mice 110603 B-NDG
B-NDG hTHPO mice 110590 B-NDG