Basic Information
-
Gene targeting strategy
-
Gene targeting strategy for B-hBST2 mice.
The exons 1-4 of mouse Bst2 gene that encode the extracellular domain were replaced by human BST2 exons 1-4 in B-hBST2 mice.
-
Protein expression analysis in spleen
-
Human CRTH2 protein expression analysis in wild-type and humanized B-hCRTH2 mice plus. Blood-derived eosinophils were isolated from wild-type C67BL/6 and B-hCRTH2 mice plus (3 female, 3 male, 13-week-old), and analyzed using an anti-human CRTH2 antibody. Human CRTH2 protein was detected in blood-derived eosinophils from B-hCRTH2 mice plus.
Strain specific FOLR1 and FOLR2 expression analysis in wild-type mice and homozygous B-hFOLR1/hFOLR2 mice by flow RT-PCR.Mouse Folr1 and Folr2 mRNA were detectable in splenocytes of wild-type (+/+) mice. Human FOLR1 and FOLR2 mRNA were detectable only in homozygous B-hFOLR1/hFOLR2 mice, but not in wild-type (+/+) mice.
