Basic Information

Strain Name
C57BL/6N–Bst2tm1(BST2)/Bcgen
Common Name
B-hBST2 mice
Background
C57BL/6N
Catalog Number
112095
NCBI Gene ID
Related Genes
BST2 (CD317, HM1.24, TETHERIN)

Gene targeting strategy

Gene targeting strategy for B-hBST2 mice.

The exons 1-4 of mouse Bst2 gene that encode the extracellular domain were replaced by human BST2 exons 1-4 in B-hBST2 mice.

Protein expression analysis in spleen


Human CRTH2 protein expression analysis in wild-type and humanized B-hCRTH2 mice plus. Blood-derived eosinophils were isolated from wild-type C67BL/6 and B-hCRTH2 mice plus (3 female, 3 male, 13-week-old), and analyzed using an anti-human CRTH2 antibody. Human CRTH2 protein was detected in blood-derived eosinophils from B-hCRTH2 mice plus.

Strain specific FOLR1 and FOLR2 expression analysis in wild-type mice and homozygous B-hFOLR1/hFOLR2 mice by flow RT-PCR.

Mouse Folr1 and Folr2 mRNA were detectable in splenocytes of wild-type (+/+) mice. Human FOLR1 and FOLR2 mRNA were detectable only in homozygous B-hFOLR1/hFOLR2 mice, but not in wild-type (+/+) mice.