Basic Information
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Gene targeting strategy
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Gene targeting strategy for B-hCRTH2 mice plus. The BAC CH17-351D3, containing human Ptgdr2 gene, was inserted into mouse genome randomly in B-hCRTH2 mice plusGene targeting strategy for B-hCRTH2 mice plus. The BAC CH17-351D3, containing human Ptgdr2 gene, was inserted into mouse genome randomly in B-hCRTH2 mice plus.
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Protein expression analysis
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Human CRTH2 protein expression analysis in wild-type and humanized B-hCRTH2 mice plus. Blood-derived eosinophils were isolated from wild-type C67BL/6 and B-hCRTH2 mice plus (3 female, 3 male, 13-week-old), and analyzed using an anti-human CRTH2 antibody. Human CRTH2 protein was detected in blood-derived eosinophils from B-hCRTH2 mice plus.
Human CRTH2 protein expression analysis in wild-type and humanized B-hCRTH2 mice plus. Blood-derived neutrophils were isolated from wild-type C67BL/6 and B-hCRTH2 mice plus (3 female, 3 male, 13-week-old), and analyzed using an anti-human CRTH2 antibody. Human CRTH2 protein was not detected in blood-derived neutrophils from B-hCRTH2 mice.
