Basic Information

Common Name
B-hBCMA MC38
Aliases
BCM, CD269, TNFRSF13A, TNFRSF17
Catalog Number
310720
Cell Type/ Disease
Colon Carcinoma
Species/ Strain
Mus musculus, C57BL/6
Notice
Biocytogen provides engineering service to generate the humanized tumor cell lines from customer’s own cell lines. Our humanized tumor cell lines are for pharmacology services ONLY.

Description

The human BCMA coding sequence was inserted into the ROSA26 locus in MC38 cells. Human BCMA is highly expressed on the surface of B-hBCMA MC38 cells.

Targeting strategy

The exogenous promoter and human BCMA coding sequence was inserted into the ROSA26 locus in murine.

Protein expression analysis

BCMA expression analysis in B-hBCMA MC38 cells by flow cytometry. Single cell suspensions from wild-type MC38 and B-hBCMA MC38 cultures were stained with species-specific anti-BCMA antibody. Human BCMA was detected on the surface of B-hBCMA MC38 cells but not wild-type MC38 cells. The 2-B01 clone of B-hBCMA MC38 cells was used for in vivo experiments.

Tumor growth curve

Subcutaneous homograft tumor growth of B-hBCMA MC38 cells. B-hBCMA MC38 cells (5×105) and wild-type MC38 cells (5×105) were subcutaneously implanted into C57BL/6 mice (female, 5-8-week-old, n=5). Tumor volume and body weight were measured twice a week. (A) Average tumor volume ± SEM. (B)  Body weight (Mean± SEM). Volume was expressed in mm3 using the formula: V=0.5 X long diameter X short diameter2. As shown in panel A, B-hBCMA MC38 cells were able to establish tumors in vivo and can be used for efficacy studies.