AACR 2022: Generation and Validation of Humanized GARP Mice for Testing Novel Anti-Human GARP Antibodies
Conclusions
- We have generated a humanized mouse model expressing GARP in place of the murine counterpart.
- mRNA and protein expression of human GARP indicates successful generation of the humanized model.
- Humanization of GARP does not change the overall development, differentiation or distribution of immune cell types, CBC profile, or liver chemistry in mice.
- Anti-GARP antibody treatment reduced tumor growth in B-hGARP mice in combination with anti-mouse PD-1, indicating that B-hGARP mice provide a powerful model for in vivo evaluation of novel combination therapies.
Related mouse model: B-hGARP mice
