Active across all known vertebrates and originally discovered in zebrafish, the Tol2 transposon system can be used to make transgenic mouse models. Via transposase catalyzation, a transposon construct can be effectively inserted before or after the Tol2 gene with a cut-and-paste mechanism. With little chance of rearrangement or modification at the target site, the Tol2 transgenic model has many potential applications. Mainly, the transgene-mediated insertions of the Tol2 system are prone to be at the A/T rich region, which makes the expression of the transgene more efficient, and can increase the rate of gene integration as well as integrate foreign genes into A/T rich regions. Compared with traditional DNA pronuclear injection, the Tol2 system uses cytoplasmic injection, which is easy to operate and has a high success rate. Using our Tol2 system, we can deliver transgenes up to 30kb in mice.
BAC Transgenic:
BAC transgenic technology is commonly used to add exogenous genes to the host species and has been widely applied to many experimental and economical organisms such as crops/livestock.
Request a Quote
Contact us today to find out how we can help advance your research with custom generated rat, mouse or cell line models.
