Basic Information
Common Name
B-hEFEMP1 MC38
Catalog Number
321926
Disease
Colon carcinoma
Organism
Mouse
Strain
C57BL/6
Tissue
Colon
Tissue types
Colon
Application
B-hEFEMP1 MC38 cells have the capability to establish tumors in vivo and can be used for efficacy studies.
Related Genes
DHRD; DRAD; FBNL; MLVT; MTLV; S1-5; FBLN3; FIBL-3
Description
The mouse Efemp1 gene was replaced by human EFEMP1 coding sequence in B-hEFEMP1 MC38 cells. Human EFEMP1 is highly expressed on the supernatant of B-hEFEMP1 MC38 cells. B-hEFEMP1 MC38 cells have the capability to establish tumors in vivo and can be used for efficacy studies.
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Gene targeting strategy
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Gene targeting strategy for B-hEFEMP1 MC38 cells. The exogenous promoter and human EFEMP1 coding sequence was inserted to replace part of murine exon 4. The insertion disrupts the endogenous murine Efemp1 gene, resulting in a non-functional transcript.
