Basic Information
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Gene targeting strategy
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Gene targeting strategy for B-hFOLR1/hFOLR2 mice. The exons 3~6 and 3’UTR of mouse Folr1 gene and the exons 2~5 of mouse Folr2 gene that encode the full-length were replaced by the exons 3-6 and 3’UTR of human FOLR1 gene and the exons 2~5 of human FOLR2 gene in B-hFOLR1/hFOLR2 mice
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mRNA expression analysis
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Human CRTH2 protein expression analysis in wild-type and humanized B-hCRTH2 mice plus. Blood-derived eosinophils were isolated from wild-type C67BL/6 and B-hCRTH2 mice plus (3 female, 3 male, 13-week-old), and analyzed using an anti-human CRTH2 antibody. Human CRTH2 protein was detected in blood-derived eosinophils from B-hCRTH2 mice plus.
Strain specific FOLR1 and FOLR2 expression analysis in wild-type mice and homozygous B-hFOLR1/hFOLR2 mice by flow RT-PCR.Mouse Folr1 and Folr2 mRNA were detectable in splenocytes of wild-type (+/+) mice. Human FOLR1 and FOLR2 mRNA were detectable only in homozygous B-hFOLR1/hFOLR2 mice, but not in wild-type (+/+) mice.
