The Difference Between Genome Editing Technology and Transgenic Technology
Both genome editing technology and transgenic techniques can alter the genome of an organism so that the desirable trait is inherited, however the mechanism of action varies between the two technologies. Genome editing uses targeted/locus specific manipulation by knocking out or replacing targeted genes, which results in model organisms with intentionally selected and desired traits. Conversely, transgenic technology randomly integrates foreign DNA into the genome of a model organism in order to introduce new traits.
Generation of transgenic animal lines through random integration can lead to several confounding effects on gene expression. Including multiple integrations per cell, activation or disruption of endogenous genes at or near the site of integration, and unstable expression of the transgene due to epigenetic modifications. These, coupled with the constitutive and often high expression levels of the transgene, can lead to unpredictable cell behavior and irreproducible/difficult to interpret results.
Targeted gene editing overcomes these limitations by introducing modifications specifically at the endogenous gene locus, which allows the effect of gain- or loss-of-function to be studied. In addition to Biocytogen’s most advanced gene targeting technology and capacity, we can meet some customers’ special needs for random insertion transgene requests.
Transgenic Technologies Available
Tol2 Transposon System:
The Tol2 transposon system allows insertion of transgene-mediated genes at the A/T rich region so that the expression of the transgene is more efficient.
BAC Transgenic:
BAC transgenic technology randomly integrates exogenous genes into the target genome, and has been widely applied to many experimental and economical organisms such as crops and livestock.
Engineered BAC Knockin
Available upon special request. Contact our gene editing experts to learn more today.
Mb-level chromosome engineering
Available upon special request. Contact our gene editing experts to learn more today.
